Morphology Kruger Strict Criteria

Abraham Morgentaler, May Y. Fung, Doria H. Harris, R. Douglas Powers and Michael M. Alper, Sperm morphology and in vitro fertilization outcome: a direct comparison of World Health Organization and strict criteria methodologies**Presented in part at the Meeting of the American Urological Association, San Antonio, Texas, May 15 to 20, 1993.

The only difference between WHO 4th and 5th criteria is based on the cut-off for normal. The SQA-V GOLD morphology algorithm was developed by assessing stained smears of semen samples under the microscope in compliance with the WHO manual guidelines for assessing normal morphology per strict criteria (Kruger).

2014) showed that normal sperm morphology, whether according to the traditional WHO approach (including borderline forms) or to the ‘strict criteria’ (excluding. to low SDF [24]. In the.

Prospective and retrospective studies were conducted to evaluate sperm morphology using strict criteria for predicting fertilization capacity in males. Severely.

The normal value for sperm morphology is reported in two ways. The World Health Organization reports the percent of normal-shaped sperm as greater than 60%. Another method is the Kruger classification.

The technician who assessed sperm morphology attended a train- ing session conducted by Dr. Thinus Kruger, who developed the criteria that are used for strict assessments of morphology.

In addition to the morphology evaluation according to strict criteria, the. The TZI was performed as described previously (WHO, 1992; Menkveld and Kruger,

Sperm characteristics were assessed with a computer assisted semen analyzer, and sperm morphology was assessed using World Health Organization and Kruger’s strict criteria. Patients with varicocele.

The only difference between WHO 4th and 5th criteria is based on the cut-off for normal. The SQA-V GOLD morphology algorithm was developed by assessing stained smears of semen samples under the microscope in compliance with the WHO manual guidelines for assessing normal morphology per strict criteria (Kruger).

Nov 5, 2017. Of 76 cycles with male factor infertility, 8% had normal morphology by the strict Kruger criteria. Nineteen per cent of those with presumed.

Semen Analysis; Sperm Penetration Assay; Anti-sperm Antibody Testing; Strict Criteria (Kruger) Morphology; Retrograde Analysis; Sperm Chromatin Integrity.

One of the most accepted definitions of normal sperm morphology is that at least 70% of the sperm should be normal (Fitzpatrick et al., 2002). While, according to the WHO and Kruger’s strict. Bull.

except for sperm morphology, which was assessed by the strict criteria of Kruger et al. 17 (with normal morphology defined as the presence of more than 14 percent sperm of normal shape), and the.

Now with this new place, they did another semen analysis, and they are telling me that his morphology is 3% and normal is 4% on the Kruger scale. Answer: Most doctors use the strict morphology as.

Using these strict morphological criteria, a normal spermatozoon is characterized. become more limited with the development of IVF and ICSI and the ability of Kruger strict morphology to detect.

Kruger (0% Normal Morphology – Strict Criteria) ‘ Some consideration of sperm following wash using semen overlaid on 80% Pureception Clsolate ruPuresperm Percoll) followed by wash in 3 ml modified HTF. — Motility — Progression — Recovery (Motile sperm recovered/Motile sperm washed)

1984), maternal serum (Kemeter and Feichtinger, 1984; Kruger et al. were evaluated for cleavage stage and scored for morphology prior to transfer, in accordance with previously described criteria.

Sperm morphology was evaluated according to the World Health Organization (WHO) criteria and Kruger’s strict criteria. After routine semen analysis, the samples were fixed separately for further examination with AO staining.

Kruger (0% Normal Morphology – Strict Criteria) ‘ Some consideration of sperm following wash using semen overlaid on 80% Pureception Clsolate ruPuresperm Percoll) followed by wash in 3 ml modified HTF. — Motility — Progression — Recovery (Motile sperm recovered/Motile sperm washed)

Sperm morphology is assessed using one of 2 sets of characteristics. Kruger criteria are strict and consider minute irregularities as abnormal. World Health.

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Semen Analysis. The most important parameters checked are sperm count, motility, morphology, and the presence of inflammation cells. Sperm count, expressed as millions per cubic millimeter, should be over 20 million. Motility, or the percentage of forward-moving sperm out of the total sperm population, should be above 50 percent.

Main Outcome Measure. Strict morphology analysis 1 month before the beginning of IUI following the criteria of Kruger et al.

Main Outcome Measure(s): Samples were evaluated for total sperm count, motlity, progression, and morphology using Kruger’s strict criteria. The ICSI cycle outcome parameters included fertilization, clinical pregnancy, implantation, live birth, and blastulation rates and blastocyst quality.

Purpose:The purpose of this study was to assess the predictive value of a modified form of Kruger's strict criteria for sperm morphology for pregnancy outcomes.

These studies aimed to compare and optimize the methods and techniques presently used to assess sperm morphology using strict criteria. Liquefied semen is the source of spermatozoa generally used to examine the percentage with normal morphology.

Genetic factors are suspectedA. Kruger’s strict criteria), and the results agree with ours and also indicate a correlation between an increase in obesity and poorer sperm morphology ( Bieniek.

A semen analysis (plural: semen analyses), also called "seminogram" evaluates certain. One source states that 30% of men with a normal semen analysis actually. Regarding sperm morphology, the WHO criteria as described in 2010 state. Haugen TB, Kruger T, Wang C, Mbizvo MT, Vogelsong KM (May–Jun 2010).

According to the strict criteria defined by Kruger et al. (1988), a normal spermatozoon has. gene with abnormal sperm head morphology in infertile men. Briefly, seminal volume was determined in.

The only difference between WHO 4th and 5th criteria is based on the cut-off for normal. The SQA-V GOLD morphology algorithm was developed by assessing stained smears of semen samples under the microscope in compliance with the WHO manual guidelines for assessing normal morphology per strict criteria (Kruger).

In a study performed by Kruger et al. (1988), they found that in their. In some men, no more than 4-5% of their spermatozoa meet the strict criteria [27] of normal head morphology. Although such.

Male partners are tested with the strict criteria sperm morphology test prior to IVF to. Menkveld R, Oettle E, Kruger T, et al: Atlas of human sperm morphology.

Arch Androl. 1992 Jan-Feb;28(1):15-7. Evaluation of sperm morphology using Kruger's strict criteria. Check JH(1), Adelson HG, Schubert BR, Bollendorf A.

strict Krüger criteria predict the fertilization rate, embryo score. Morphology in human spermatozoa according to Krüger's strict criteria, WHO criteria and the TZI.

Four measurements were done including the middle piece, head length- head width and tail length for 200 spermatozoa with normal morphology. Comparisons were made between the stains that which showed a better morphology and which is more appropriate for.

Aug 16, 2017. Sperm morphology tests examine semen samples under a microscope and calculate the percentage of sperm with a normal form (NF) in the.

Abstract. Although sperm morphology evaluation is one of the most important aspects of the semen analysis if done correctly and accurately, a trend is developing in which many laboratories or clinicians no longer regard sperm morphology as relevant due to the very low normal reference value of only 4% morphological normal spermatozoa given in.

Sperm morphology was evaluated in a single sample for the initial assessment of patients, count- ing 100 cells, according to the strict criteria proposed by Kruger/Tygerberg and adopted by the World Health Organization (10), using Papanicolaou stain.

Dichotomous outcomes were used for the following semen analyses parameters: motility (less than 50% motile compared with 50% motile or higher), morphology (strict Kruger criteria: less than 5%.

The aim of this study was to investigate the correlation between strict sperm morphology determined using Kruger's strict criteria and the outcome of ICSI in IVF.

Sperm morphology was evaluated in a single sample for the initial assessment of patients, count- ing 100 cells, according to the strict criteria proposed by Kruger/Tygerberg and adopted by the World Health Organization (10), using Papanicolaou stain.

The evaluation of sperm morphology was performed according to the criteria normally used in the different laboratories: WHO (1992) criteria for laboratory A, and Tygerberg strict criteria for.

1992; World Health Organization, 1999). Sperm morphology was scored using the Tygerberg Kruger strict criteria (Kruger et al., 1987). The most important role of CASA at this time seems to be.

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A study was included if it 1) reported ultrasound verified clinical pregnancies per IUI cycle, 2) assessed sperm morphology using the Kruger strict criteria prior to any wash, processing or selection process, 3) assessed a minimum of 200 spermatozoa in each sperm morphology analysis, and 4) reported sperm morphology at the greater than 4% and 4% or.

The strict criteria of sperm morphology (e.g. Kruger's criteria) use the examination of spermatozoa that had penetrated cervical mucus for the definition of normal.

Aug 2, 2017. Find out how sperm morphology can affect fertility. but we use a strict Kruger morphology that means 14 percent or more is normal range.

Advantages of strict (Tygerberg) criteria for evaluation of sperm morphology. Quantitatively, the sperm should have a head length of 3 to 5 µm and width of 2 to 3 µm, as well as a head width to length ratio of between three-fifths and two-thirds, with a tail measuring about 45 µm in length [48].

including the World Health Organization [WHO] and strict morphological criteria in a fertile and subfertile population, has been described [Menkveld et al. 2001]. Computer-assisted semen analysis.

Archives of Andrology Journal of Reproductive Systems. Evaluation of Sperm Morphology Using Kruger’s Strict Criteria References; Citations. Prospective and retrospective studies were conducted to evaluate sperm morphology using strict criteria for predicting fertilization capacity in males. Severely impaired male fertility potential was.

Of all semen parameters, sperm morphology turns out to be the best predictor of a man’s fertilizing potential (Bostofte et al, 1982; Rogers et al, 1983; Kruger et al. This differs from the strict.

The most clinically useful morphology criteria is the Kruger “Strict” morphology ( which as mentioned above, is the same as the WHO 5th edition criteria).

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In vitro fertilization (IVF) resulted in 16% pregnancies per attempt, but the effective cumulative pregnancy rate. strict’ criteria (Kruger et al., 1986Kruger et al., , 1988 WHO, 1999) (Table I),

Sep 30, 2016. In couples with infertility, abnormal strict morphology of 0% normal forms. Strict morphology was evaluated using the Kruger strict criteria.

strict criteria for the evaluation of sperm morphology. the evolution of sperm morphology evaluation criteria in. Similarly, in 1986 and 1988, Kruger et al.

1996), computer-assisted semen analysis (CASA) was used in 49 men before and after varicocelectomy; all sperm parameters of count, motility and morphology were improved. according to Kruger’s.